Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer-peptide conjugate

Chem Commun (Camb). 2012 May 16;48(39):4740-2. doi: 10.1039/c2cc30536d. Epub 2012 Apr 5.

Abstract

When a biotinylated FRET probe based on a peptide-thrombin binding aptamer conjugate was introduced together with streptavidin and biotinylated nuclear export signal peptide into HeLa cells, the resulting ternary complex enabled visualization of K(+) concentration changes in the cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Ions
  • Peptides / chemistry*
  • Potassium / analysis
  • Potassium / chemistry*
  • Streptavidin / chemistry

Substances

  • Aptamers, Nucleotide
  • Fluorescent Dyes
  • Ions
  • Peptides
  • thrombin aptamer
  • Streptavidin
  • Potassium