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Ophthalmic Res. 2012;48(2):59-66. doi: 10.1159/000335365.

Comparing the effects of two different irrigation solutions on an isolated perfused vertebrate retina.

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1
Department of Ophthalmology I, Eberhard Karls University of Tübingen, Tübingen, Germany.

Abstract

PURPOSE:

To compare the effect of a taurine-containing intraocular irrigation solution (PuriProtect TM) to a standard irrigation solution (BSS TM) we evaluated the retinal function using an electroretinogram (ERG) and analyzed the survival of retinal ganglion cells on isolated whole mount retinas.

MATERIALS AND METHODS:

During ERG recordings, each irrigation solution was superfused for 45 min with the relevant irrigation solution. To investigate the effects on photoreceptor function, 1 m M asparate was added to obtain a-waves.The recovery of the a- and b-wave was monitored after superfusing the retinas with standard medium again. To evaluate the percentage of dead ganglion cells, retinas were stored for 24 h at 4°C in darkness and after staining the retinas with ethidium homodimer-1 the retinas were analyzed using fluorescence microscopy.

RESULTS:

The application of standard medium supplemented with 2 m M taurine resulted in a significant increase of the b-wave amplitude compared to standard medium alone. The a-wave amplitudes showed no significant changes under taurine supplementation. Compared to standard medium BSS showed no significant decrease in b-wave amplitudes, but a significant decrease ina-wave amplitudes. In contrast to BSS there were no significant changes in the a- or b-wave amplitudes detectable after the application of PuriProtect. At the end of the washout period no significant changes in a- or b-wave amplitudes were recorded for any tested irrigation solution. Retinas stored for 24 h in PuriProtect or in standard medium with taurine had a statistically significant smaller amount of dead cells than retinas stored in standard medium without taurine supplementation.

CONCLUSIONS:

BSS does not seem to be an ideal irrigation solution, because it compromises the a-wave in the ERG. In contrast to BSS, PuriProtect showed no significant impact on the ERG and showed a better long-term effect on ganglion cell survival. Taurine supplementation,therefore, seems to be neuroprotective and its supplementation to an intraocular irrigation solution favorable for the retina.

PMID:
22472946
DOI:
10.1159/000335365
[Indexed for MEDLINE]
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