Format

Send to

Choose Destination
Viruses. 2012 Feb;4(2):211-35. doi: 10.3390/v4020211. Epub 2012 Feb 3.

Back to BAC: the use of infectious clone technologies for viral mutagenesis.

Author information

1
School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, Australia. r.hall4@uq.edu.au

Abstract

Bacterial artificial chromosome (BAC) vectors were first developed to facilitate the propagation and manipulation of large DNA fragments in molecular biology studies for uses such as genome sequencing projects and genetic disease models. To facilitate these studies, methodologies have been developed to introduce specific mutations that can be directly applied to the mutagenesis of infectious clones (icBAC) using BAC technologies. This has resulted in rapid identification of gene function and expression at unprecedented rates. Here we review the major developments in BAC mutagenesis in vitro. This review summarises the technologies used to construct and introduce mutations into herpesvirus icBAC. It also explores developing technologies likely to provide the next leap in understanding these important viruses.

KEYWORDS:

DNA viruses; artificial; bacterial; chromosomes; cloning; genetic; infectious clone; molecular methods; mutagenesis; recombination; transposition

PMID:
22470833
PMCID:
PMC3315213
DOI:
10.3390/v4020211
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center