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PLoS One. 2012;7(3):e34322. doi: 10.1371/journal.pone.0034322. Epub 2012 Mar 28.

Overexpression of DNA polymerase zeta reduces the mitochondrial mutability caused by pathological mutations in DNA polymerase gamma in yeast.

Author information

1
Department of Genetics, Biology of Microorganisms, Anthropology, Evolution, University of Parma, Parma, Italy. enrico.baruffini@nemo.unipr.it

Abstract

In yeast, DNA polymerase zeta (Rev3 and Rev7) and Rev1, involved in the error-prone translesion synthesis during replication of nuclear DNA, localize also in mitochondria. We show that overexpression of Rev3 reduced the mtDNA extended mutability caused by a subclass of pathological mutations in Mip1, the yeast mitochondrial DNA polymerase orthologous to human Pol gamma. This beneficial effect was synergistic with the effect achieved by increasing the dNTPs pools. Since overexpression of Rev3 is detrimental for nuclear DNA mutability, we constructed a mutant Rev3 isoform unable to migrate into the nucleus: its overexpression reduced mtDNA mutability without increasing the nuclear one.

PMID:
22470557
PMCID:
PMC3314619
DOI:
10.1371/journal.pone.0034322
[Indexed for MEDLINE]
Free PMC Article

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