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Neurosci Res. 2013 Jan;75(1):59-64. doi: 10.1016/j.neures.2012.03.009. Epub 2012 Mar 24.

Parallel and patterned optogenetic manipulation of neurons in the brain slice using a DMD-based projector.

Author information

1
Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai 980-8577, Japan.

Abstract

Optical manipulation technologies greatly advanced the understanding of the neuronal network and its dysfunctions. To achieve patterned and parallel optical switching, we developed a microscopic illumination system using a commercial DMD-based projector and a software program. The spatiotemporal patterning of the system was evaluated using acute slices of the hippocampus. The neural activity was optically manipulated, positively by the combination of channelrhodopsin-2 (ChR2) and blue light, and negatively by the combination of archaerhodopsin-T (ArchT) and green light. It is suggested that our projector-managing optical system (PMOS) would effectively facilitate the optogenetic analyses of neurons and their circuits.

PMID:
22469653
DOI:
10.1016/j.neures.2012.03.009
[Indexed for MEDLINE]

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