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World J Gastroenterol. 2012 Mar 21;18(11):1257-61. doi: 10.3748/wjg.v18.i11.1257.

Bacterial flora concurrent with Helicobacter pylori in the stomach of patients with upper gastrointestinal diseases.

Author information

1
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Abstract

AIM:

To investigate the non-Helicobacter pylori (H. pylori) bacterial flora concurrent with H. pylori infection.

METHODS:

A total of 103 gastric biopsy specimens from H. pylori positive patients were selected for bacterial culture. All the non-H. pylori bacterial isolates were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).

RESULTS:

A total of 201 non-H. pylori bacterial isolates were cultivated from 67 (65.0%) of the 103 gastric samples, including 153 isolates identified successfully at species level and 48 at genus level by MALDI-TOF MS. The dominant species were Streptococcus, Neisseria, Rothia and Staphylococcus, which differed from the predominantly acid resistant species reported previously in healthy volunteers. The prevalence of non-H. pylori bacteria was higher in non-ulcer dyspepsia group than in gastric ulcer group (100% vs 42.9%, P < 0.001). Six bacterial species with urease activity (Staphylococcus epidermidis, Staphylococcus warneri, Staphylococcus capitis, Staphylococcus aureus, Brevibacterium spp. and Klebsiella pneumoniae) were also isolated.

CONCLUSION:

There is a high prevalence of the non-H. pylori bacteria concurrent with H. pylori infection, and the non-H. pylori bacteria may also play important as-yet-undiscovered roles in the pathogenesis of stomach disorders.

KEYWORDS:

Bacterial flora; Gastrointestinal diseases; Matrix-assisted laser desorption ionization time-of-flight mass spectrometry; Non-Helicobacter pylori

PMID:
22468090
PMCID:
PMC3309916
DOI:
10.3748/wjg.v18.i11.1257
[Indexed for MEDLINE]
Free PMC Article

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