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Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6094-9. doi: 10.1073/pnas.1201288109. Epub 2012 Mar 30.

Structural characterization of mRNA-tRNA translocation intermediates.

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  • 1Structural Biology Unit, CIC-bioGUNE, Bizkaia Technology Park, Derio 48160, Basque Country, Spain.

Abstract

Cryo-EM analysis of a wild-type Escherichia coli pretranslocational sample has revealed the presence of previously unseen intermediate substates of the bacterial ribosome during the first phase of translocation, characterized by intermediate intersubunit rotations, L1 stalk positions, and tRNA configurations. Furthermore, we describe the domain rearrangements in quantitative terms, which has allowed us to characterize the processivity and coordination of the conformational reorganization of the ribosome, along with the associated changes in tRNA ribosome-binding configuration. The results are consistent with the view of the ribosome as a molecular machine employing Brownian motion to reach a functionally productive state via a series of substates with incremental changes in conformation.

PMID:
22467828
PMCID:
PMC3340995
DOI:
10.1073/pnas.1201288109
[PubMed - indexed for MEDLINE]
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