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J Proteomics. 2012 Aug 30;75(16):5014-5026. doi: 10.1016/j.jprot.2012.03.015. Epub 2012 Mar 20.

Probing neuropeptide signaling at the organ and cellular domains via imaging mass spectrometry.

Author information

1
School of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705-2222, USA.
2
Department of Chemistry, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705-2222, USA.
3
School of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705-2222, USA; Department of Chemistry, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705-2222, USA. Electronic address: lli@pharmacy.wisc.edu.

Abstract

Imaging mass spectrometry (IMS) has evolved to be a promising technology due to its ability to detect a broad mass range of molecular species and create density maps for selected compounds. It is currently one of the most useful techniques to determine the spatial distribution of neuropeptides in cells and tissues. Although IMS is conceptually simple, sample preparation steps, mass analyzers, and software suites are just a few of the factors that contribute to the successful design of a neuropeptide IMS experiment. This review provides a brief overview of IMS sampling protocols, instrumentation, data analysis tools, technological advancements and applications to neuropeptide localization in neurons and endocrine tissues. Future perspectives in this field are also provided, concluding that neuropeptide IMS would greatly facilitate studies of neuronal network and biomarker discovery.

PMID:
22465716
PMCID:
PMC3389296
DOI:
10.1016/j.jprot.2012.03.015
[Indexed for MEDLINE]
Free PMC Article

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