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Mol Cell Probes. 2012 Jun;26(3):113-5. doi: 10.1016/j.mcp.2012.03.005. Epub 2012 Mar 17.

Highly sensitive detection of alkaline phosphatase using molecular beacon probes based on enzymatic polymerization.

Author information

1
School of Biological Science and Technology, Central South University, Changsha 410013, China. lhlmcb@yahoo.com.cn

Abstract

We have developed a new methodology for highly sensitive alkaline phosphatase assay using molecular beacon probes. No incubation step is needed to obtain a limit of detection for ALP of 2×10(-16) M. Furthermore, ALP inhibition by the inhibitor okadaic acid is shown, demonstrating the potential for high-throughput screening for inhibitors.

PMID:
22464925
DOI:
10.1016/j.mcp.2012.03.005
[Indexed for MEDLINE]

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