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Br J Nutr. 2012 Dec 28;108(12):2148-57. doi: 10.1017/S0007114512000396. Epub 2012 Apr 2.

Postprandial expression of growth-related genes in Atlantic salmon (Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation.

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  • 1CIMAR/CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental and ICBAS - Instituto de Ciências Biomédicas de Abel Salazar, Universidade de Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal. lvalente@icbas.up.pt

Abstract

We investigated postprandial changes in transcript abundance following a single satiating meal in juvenile Atlantic salmon (Salmo salar L.) (about 70 g body mass) following fasting for 1 week at 12°C. The expression of twenty-three growth-related genes was determined in fast myotomal muscle using quantitative real-time PCR at the following postprandial time points: - 12, 0, 1, 3, 6, 12, 24, 48 and 96 h. The gut was fullest 1-6 h after feeding and emptied within 48-96 h. IGF-I, MyoD1c, MRF4 and myf5 transcripts were sharply up-regulated within 1 h of refeeding and are promising candidate genes involved in a fast-response signalling system that regulates fish myotomal muscle growth. These genes clustered together with MyoD1b and suggest a coordinated regulation to favour resumption of myogenesis as an early response to feeding. Insulin-like growth factor (IGF)-II and the ubiquitin ligase MAFbx/atrogin-1 were initially down-regulated but restored to initial values after 12 h. It is also suggested that local production of IGF-I within the muscle might suppress catabolic pathways depressing MAFbx/atrogin-1.

PMID:
22464448
DOI:
10.1017/S0007114512000396
[PubMed - indexed for MEDLINE]
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