Send to

Choose Destination
Microb Biotechnol. 2013 Jan;6(1):17-26. doi: 10.1111/j.1751-7915.2012.00342.x. Epub 2012 Mar 29.

Evolution of Bacillus thuringiensis Cry toxins insecticidal activity.

Author information

Instituto de Biotecnología, Universidad Nacional Autónoma de México. Apdo. postal 510-3, Cuernavaca 62250, Morelos, Mexico.


Insecticidal Cry proteins produced by Bacillus thuringiensis are use worldwide in transgenic crops for efficient pest control. Among the family of Cry toxins, the three domain Cry family is the better characterized regarding their natural evolution leading to a large number of Cry proteins with similar structure, mode of action but different insect specificity. Also, this group is the better characterized regarding the study of their mode of action and the molecular basis of insect specificity. In this review we discuss how Cry toxins have evolved insect specificity in nature and analyse several cases of improvement of Cry toxin action by genetic engineering, some of these examples are currently used in transgenic crops. We believe that the success in the improvement of insecticidal activity by genetic evolution of Cry toxins will depend on the knowledge of the rate-limiting steps of Cry toxicity in different insect pests, the mapping of the specificity binding regions in the Cry toxins, as well as the improvement of mutagenesis strategies and selection procedures.

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center