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J Biomed Opt. 2012 Feb;17(2):026003. doi: 10.1117/1.JBO.17.2.026003.

Three-dimensional differential interference contrast microscopy using synthetic aperture imaging.

Author information

1
Korea University, Department of Physics, Seoul 136-701, Republic of Korea.

Abstract

We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.

PMID:
22463035
PMCID:
PMC3380814
DOI:
10.1117/1.JBO.17.2.026003
[Indexed for MEDLINE]
Free PMC Article
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