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Am J Physiol Gastrointest Liver Physiol. 2012 Jun 15;302(12):G1359-63. doi: 10.1152/ajpgi.00493.2011. Epub 2012 Mar 29.

A nomenclature for intestinal in vitro cultures.

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1
UCLA/VA Greater Los Angeles, 11301 Wilshire Blvd. (10H2) Los Angeles, CA 90073, USA. stelzner@ucla.edu

Abstract

Many advances have been reported in the long-term culture of intestinal mucosal cells in recent years. A significant number of publications have described new culture media, cell formations, and growth patterns. Furthermore, it is now possible to study, e.g., the capabilities of isolated stem cells or the interactions between stem cells and mesenchyme. However, at the moment there is significant variation in the way these structures are described and named. A standardized nomenclature would benefit the ability to communicate and compare findings from different laboratories using the different culture systems. To address this issue, members of the NIH Intestinal Stem Cell Consortium herein propose a systematic nomenclature for in vitro cultures of the small and large intestine. We begin by describing the structures that are generated by preparative steps. We then define and describe structures produced in vitro, specifically: enterosphere, enteroid, reconstituted intestinal organoid, induced intestinal organoid, colonosphere, colonoid, and colonic organoid.

PMID:
22461030
PMCID:
PMC3378093
DOI:
10.1152/ajpgi.00493.2011
[Indexed for MEDLINE]
Free PMC Article
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