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Med Sci Monit. 2012 Apr;18(4):HY5-8.

A novel asymmetric-loop molecular beacon-based two-phase hybridization assay for accurate and high-throughput detection of multiple drug resistance-conferring point mutations in Mycobacterium tuberculosis.

Author information

1
Clinical Experimental Laboratory of Biosensor and Microarray, and Center of Molecular and Genetic Diagnosis, Southwest Hospital, 3rd Military Medical University, Chongqing, PR China.

Abstract

The accurate and high-throughput detection of drug resistance-related multiple point mutations remains a challenge. Although the combination of molecular beacons with bio-immobilization technology, such as microarray, is promising, its application is difficult due to the ineffective immobilization of molecular beacons on the chip surface. Here, we propose a novel asymmetric-loop molecular beacon in which the loop consists of 2 parts. One is complementary to a target, while the other is complementary to an oligonucleotide probe immobilized on the chip surface. With this novel probe, a two-phase hybridization assay can be used for simultaneously detecting multiple point mutations. This assay will have advantages, such as easy probe availability, multiplex detection, low background, and high-efficiency hybridization, and may provide a new avenue for the immobilization of molecular beacons and high-throughput detection of point mutations.

PMID:
22460100
PMCID:
PMC3560835
[Indexed for MEDLINE]
Free PMC Article
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