Objective: To establish HPLC fingerprint of Herba Veronicae Dilatatae.
Methods: Beckman C18 column was used,with mixtures of 0.5% ice acetic acid-methanol as mobile phase. The flow rate was 1 mL/min. The wavelength of detection was 254 nm.
Results: After the mutual mode of HPLC fingerprint was set up, comparsion of similar degree with the medicinal materials of different batch were carried out.
Conclusion: According to the selected chromatographic conditions, a good fingerprint has been described. The method is simple, accurate and reproducible. It may have practical value for the quality control of samples of Herba Veronicae Dilatatae.