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Bioanalysis. 2012 Mar;4(6):675-84. doi: 10.4155/bio.12.11.

Analytical bias between species caused by matrix effects in quantitative analysis of a small-molecule pharmaceutical candidate in plasma.

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1
Covance, Alnwick, Northumberland, UK.

Abstract

BACKGROUND:

Suppression or enhancement of MS ionization, particularly evident when electrospray is used as the source of ions, has been widely discussed.

METHODS:

An assay for a small-molecule pharmaceutical in dog plasma between 1-300 ng/ml was validated with a mean bias across the calibration range of 5.0%. When the calibration sample matrix was substituted for human plasma, the mean bias across the range increased to 29.1%. A study of bias originating as a result of matrix effects, arising from endogenous glycerophosphocholine species, in plasma sources is discussed.

CONCLUSION:

A simple strategy to assess the potential of any unmitigated matrix effect to bias quantitative analysis by nonequivalent ionization induction or suppression is evaluated.

PMID:
22452258
DOI:
10.4155/bio.12.11
[Indexed for MEDLINE]
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