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J Biotechnol. 2012 Aug 31;160(3-4):195-201. doi: 10.1016/j.jbiotec.2012.03.006. Epub 2012 Mar 17.

Calorimetric control of the specific growth rate during fed-batch cultures of Saccharomyces cerevisiae.

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University of Applied Sciences Esslingen, Department of Natural Sciences, Kanalstrasse 33, 73728 Esslingen, Germany.


The specific growth rate of a Saccharomyces cerevisiae strain with glucose as limiting C-source was estimated from the measured heat flow produced by the cells. For the cultivation a standard 30 l laboratory bioreactor was used, which was extended in such a way that heat balancing is possible. The feed rate was adjusted by a feedforward/feedback controller such that the specific growth rate was kept on the desired set-point value. On the basis of experimental investigations it was demonstrated that the specific growth rate can be controlled at a given set point value below the critical value to prevent the production of growth-inhibitory ethanol due to the Crabtree effect. With this control strategy high biomass concentrations of more than 110 g l(-1) can be obtained.

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