Format

Send to

Choose Destination
Appl Spectrosc. 2012 Apr;66(4):447-50. doi: 10.1366/11-06426.

Selection of haploid maize kernels from hybrid kernels for plant breeding using near-infrared spectroscopy and SIMCA analysis.

Author information

1
Ames Laboratory-USDOE, Iowa State University, Ames, Iowa 50011, USA. jonesrw@ameslab.gov

Abstract

Samples of haploid and hybrid seed from three different maize donor genotypes after maternal haploid induction were used to test the capability of automated near-infrared transmission spectroscopy to individually differentiate haploid from hybrid seeds. Using a two-step chemometric analysis in which the seeds were first classified according to genotype and then the haploid or hybrid status was determined proved to be the most successful approach. This approach allowed 11 of 13 haploid and 25 of 25 hybrid kernels to be correctly identified from a mixture that included seeds of all the genotypes.

PMID:
22449327
DOI:
10.1366/11-06426
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center