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Nat Chem Biol. 2012 Mar 25;8(5):434-6. doi: 10.1038/nchembio.921.

An engineered eukaryotic protein glycosylation pathway in Escherichia coli.

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1
School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York, USA.

Abstract

We performed bottom-up engineering of a synthetic pathway in Escherichia coli for the production of eukaryotic trimannosyl chitobiose glycans and the transfer of these glycans to specific asparagine residues in target proteins. The glycan biosynthesis was enabled by four eukaryotic glycosyltransferases, including the yeast uridine diphosphate-N-acetylglucosamine transferases Alg13 and Alg14 and the mannosyltransferases Alg1 and Alg2. By including the bacterial oligosaccharyltransferase PglB from Campylobacter jejuni, we successfully transferred glycans to eukaryotic proteins.

PMID:
22446837
PMCID:
PMC3449280
DOI:
10.1038/nchembio.921
[Indexed for MEDLINE]
Free PMC Article
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