Reading heteromic DNA. (a) Example event for heteromer DNA 1. Unzipping and synthesis phases are indicated. (b) Demonstration of the repeatability and similarity of current levels found in multiple events. Time-ordered levels for n = 20 events collected on N = 2 pores are found using a level detection algorithm. A consensus current level sequence was created from multiple events. The time-ordered levels consistent with the consensus sequence are overlaid (one color for each event). The symmetry of the plot about level ± 1 shows that the same levels occur during both unzipping and synthesis (in reverse order). The correlated sequence is shown below. Levels not detected, owing to repeat nucleotides and/or similar currents, are indicated with the unobserved nucleotide beneath the sequence. (c) s.d. derived from the scatter of each overlaid level. The average 1-sigma fluctuation in levels is below 1% of Io. (d) The probability, Pobs, of finding a given level in the proper order. Some levels, such as level 10 or level 21 are found in all events (n = 20). Levels immediately before the transition from unzipping to synthesis are often too fast to be detected, resulting in low Pobs.