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Curr Opin Microbiol. 2012 Jun;15(3):364-72. doi: 10.1016/j.mib.2012.02.005. Epub 2012 Mar 21.

Global relative and absolute quantitation in microbial proteomics.

Author information

1
Institute for Microbiology, Ernst Moritz Arndt University Greifswald, F-L-Jahn-Strasse 15, 17487 Greifswald, Germany.

Abstract

Proteomic studies are designed to yield either qualitative information on proteins (identification, distribution, posttranslational modifications, interactions, structure and function) or quantitative information (abundance, distribution within different localizations, temporal changes in abundance due to synthesis and degradation or both). To this end these studies can draw upon a wide range of qualitative and quantitative gel-based and gel-free techniques. This review summarizes current proteomic workflows for global relative or absolute protein quantitation and their application in microbial physiology.

PMID:
22445110
DOI:
10.1016/j.mib.2012.02.005
[Indexed for MEDLINE]

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