Format

Send to

Choose Destination
See comment in PubMed Commons below
Anal Chem. 2012 May 1;84(9):4014-21. doi: 10.1021/ac203330z. Epub 2012 Apr 19.

Compartment modeling for mammalian protein turnover studies by stable isotope metabolic labeling.

Author information

1
Department of Pharmaceutical Chemistry and Mass Spectrometry Facility, University of California, San Francisco, California 94158-2517, USA. sguan@cgl.ucsf.edu

Abstract

Protein turnover studies on a proteome scale based on metabolic isotopic labeling can provide a systematic understanding of mechanisms for regulation of protein abundances and their transient behaviors. At this time, these large-scale studies typically utilize a simple kinetic model to extract protein dynamic information. Although many high-quality, protein isotope incorporation data are available from those experiments, accurate and additionally useful protein dynamic information cannot be extracted from the experimental data by use of the simple kinetic models. In this paper, we describe a formal connection between data obtained from elemental isotope labeling experiments and the well-known compartment modeling, and we demonstrate that an appropriate application of a compartment model to turnover of proteins from mammalian tissues can indeed lead to a better fitting of the experimental data.

PMID:
22444387
PMCID:
PMC3923578
DOI:
10.1021/ac203330z
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society Icon for PubMed Central
    Loading ...
    Support Center