Format

Send to

Choose Destination
Chembiochem. 2012 May 7;13(7):959-63. doi: 10.1002/cbic.201200045. Epub 2012 Mar 20.

A universal expression tag for structural and functional studies of proteins.

Author information

1
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Strasse 9, 60438 Frankfurt, Germany. rogov@bpc.uni-frankfurt.de

Abstract

Modified ubiquitin sequences, each completed with a His tag and a TEV cleavage site, were designed to enhance the expression of protein/peptide targets. With this new system we have been able to characterize several peptide-protein interactions by ITC and by NMR and CD spectroscopic methods, including the interactions of LIR domains with autophagy modifiers.

PMID:
22434781
DOI:
10.1002/cbic.201200045
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center