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J Vasc Res. 2012;49(3):198-206. doi: 10.1159/000332959. Epub 2012 Mar 14.

Expression of a disintegrin and metalloprotease in human abdominal aortic aneurysms.

Author information

1
Clinic of Vascular Surgery, Institute of Pathology, Department of Nephrology, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany.

Abstract

OBJECTIVES:

The a disintegrin and metalloprotease (ADAM) family of metalloproteases possesses a proteolytic function and activates various inflammatory factors. Their expression pattern in an abdominal aortic aneurysm (AAA) is as yet unknown. The aim of this study was to make a detailed analysis of the expression of ADAMs 8, 9, 10, 12, 15 and 17, and their tissue inhibitors of metalloprotease (TIMP)-1 and TIMP-3 in patients with AAA.

DESIGN:

The aortic vessel walls of AAA patients (n=20) and non-aneurysmal aortic specimens (n=10) were obtained by conventional surgical repair and autopsy. SYBR green-based real-time PCR, histology and immunohistochemistry were performed on all samples.

MAIN OUTCOME MEASURES:

Quantitative expression analysis and the localisation of various ADAMs in AAA.

RESULTS:

ADAMs tested in our study were expressed in both AAA and control aorta without any significant differences between the groups. In contrast, expression of TIMP-1 was significantly reduced in AAA compared to control vessels. Smooth muscle cells (SMCs), neovessels and macrophages were positive for all ADAMs and TIMPs tested. Infiltrates were negative for TIMP-3, and luminal endothelial cells were positive for ADAMs 15 and 17. A significant positive correlation was observed between ADAMs 10, 12, 15, 17, TIMP-3 and SMCs.

CONCLUSION:

ADAMs are constitutively expressed in normal aortic vessel walls and AAA, particularly in SMCs.

PMID:
22433495
DOI:
10.1159/000332959
[Indexed for MEDLINE]
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