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PLoS One. 2012;7(3):e33060. doi: 10.1371/journal.pone.0033060. Epub 2012 Mar 13.

Topical application of activity-based probes for visualization of brain tumor tissue.

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1
Department of Radiology, National Foundation for Cancer Research Center for Molecular Imaging at Case Western Reserve University, Cleveland, Ohio, United States of America.

Abstract

Several investigators have shown the utility of systemically delivered optical imaging probes to image tumors in small animal models of cancer. Here we demonstrate an innovative method for imaging tumors and tumor margins during surgery. Specifically, we show that optical imaging probes topically applied to tumors and surrounding normal tissue rapidly differentiate between tissues. In contrast to systemic delivery of optical imaging probes which label tumors uniformly over time, topical probe application results in rapid and robust probe activation that is detectable as early as 5 minutes following application. Importantly, labeling is primarily associated with peri-tumor spaces. This methodology provides a means for rapid visualization of tumor and potentially infiltrating tumor cells and has potential applications for directed surgical excision of tumor tissues. Furthermore, this technology could find use in surgical resections for any tumors having differential regulation of cysteine cathepsin activity.

PMID:
22427947
PMCID:
PMC3302795
DOI:
10.1371/journal.pone.0033060
[Indexed for MEDLINE]
Free PMC Article
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