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J Clin Microbiol. 2012 Jun;50(6):2075-8. doi: 10.1128/JCM.06548-11. Epub 2012 Mar 14.

Simultaneous identification of 29 prevalent invasive pneumococcal serotypes or pairs of serotypes by hybridization-ligation PCR.

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1
National Centre of Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.

Abstract

A hybridization-ligation PCR assay was developed for the simultaneous detection and identification of 21 pneumococcal serotypes and 8 pairs of serotypes in the same serogroup: 1, 2, 3, 4, 5, 6A, 6B, 6C-6D, 7F-7A, 8, 9A-9V, 9N-9L, 11A, 14, 15B-15C, 16F, 17F, 18B-18C, 19A, 19F, 20, 21, 22A-22F, 23A, 23B, 23F, 28A-28F, 35B and 38. This novel assay was validated with 185 serotyped pneumococcal invasive clinical isolates and 57 culture-negative pleural fluids previously typed by real-time PCR.

PMID:
22422847
PMCID:
PMC3372161
DOI:
10.1128/JCM.06548-11
[Indexed for MEDLINE]
Free PMC Article
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