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Int J Biol Sci. 2012;8(4):451-8. doi: 10.7150/ijbs.4038. Epub 2012 Mar 3.

The silencing of RECK gene is associated with promoter hypermethylation and poor survival in hepatocellular carcinoma.

Author information

1
Clinical Oncology Laboratory, Changzhou Tumor Hospital, Soochow University, Changzhou, China. zhangchangsong@suda.edu.cn

Abstract

BACKGROUND:

To evaluate the promoter methylation status of RECK gene and mRNA expression in patients with hepatocellular carcinoma (HCC).

METHODS:

We analyzed RECK methylation by MSP, and RECK mRNA by real-time PCR in 74 HCC. The liver cell lines (7721, Chang and Hep-G2) were treated with 5-Aza-CdR and TSA.

RESULTS:

RECK mRNA were lower in HCC tissues (Mean (-∆Ct) = -3.29) than that in Non-Hcc tissues (Mean (-∆Ct) = -2.42). Expression of RECK was elevated in only 24 (32.43%) of the 74 HCC patients but decreased (-∆∆Ct<0) in 50 (67.57%) of the patients. RECK promoter was hypermethylated in 55.4% (41/74) of HCCs, and in only 17.6% (13/74) of Non-Hcc samples. RECK mRNA were lower in HCC patients with hypermethylation (∆MI>=0.5) (Mean (-∆∆Ct) = -1.75) than those with demethylation (∆MI<0.5) (Mean (-∆∆Ct) = 0.05), and there is a decreased tendency for RECK mRNA in HCC patients with promoter hypermethylation (p = 0.002). There was a significantly correlation found between RECK mRNA and poor survival after surgery. After treated by 5-Aza-CdR and TSA, we found that RECK mRNA induced different changes in 7721, Chang and Hep-G2 cells. And RECK demethylation also induced by epigenetic inhibitors.

CONCLUSION:

The results suggested that the hypermethylation may lead to promoter silencing of RECK mRNA and associated with poor survival in HCC.

KEYWORDS:

RECK; hepatocellular carcinoma; methylation.

PMID:
22419890
PMCID:
PMC3303171
DOI:
10.7150/ijbs.4038
[Indexed for MEDLINE]
Free PMC Article

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