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Sci Rep. 2012;2:311. doi: 10.1038/srep00311. Epub 2012 Mar 13.

A bio-catalytic approach to aliphatic ketones.

Author information

1
Department of Chemical Engineering and Materials Science, University of Minnesota, Twin Cities, Minnesota 55455, USA.

Abstract

Depleting oil reserves and growing environmental concerns have necessitated the development of sustainable processes to fuels and chemicals. Here we have developed a general metabolic platform in E. coli to biosynthesize carboxylic acids. By engineering selectivity of 2-ketoacid decarboxylases and screening for promiscuous aldehyde dehydrogenases, synthetic pathways were constructed to produce both C5 and C6 acids. In particular, the production of isovaleric acid reached 32 g/L (0.22 g/g glucose yield), which is 58% of the theoretical yield. Furthermore, we have developed solid base catalysts to efficiently ketonize the bio-derived carboxylic acids such as isovaleric acid and isocaproic acid into high volume industrial ketones: methyl isobutyl ketone (MIBK, yield 84%), diisobutyl ketone (DIBK, yield 66%) and methyl isoamyl ketone (MIAK, yield 81%). This hybrid "Bio-Catalytic conversion" approach provides a general strategy to manufacture aliphatic ketones, and represents an alternate route to expanding the repertoire of renewable chemicals.

PMID:
22416247
PMCID:
PMC3301443
DOI:
10.1038/srep00311
[Indexed for MEDLINE]
Free PMC Article

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