Format

Send to

Choose Destination
See comment in PubMed Commons below
Bioessays. 2012 May;34(5):427-36. doi: 10.1002/bies.201100148. Epub 2012 Mar 13.

Image analysis in fluorescence microscopy: bacterial dynamics as a case study.

Author information

1
Princeton University, Department of Molecular Biology, Princeton, NJ, USA.

Abstract

Fluorescence microscopy is the primary tool for studying complex processes inside individual living cells. Technical advances in both molecular biology and microscopy have made it possible to image cells from many genetic and environmental backgrounds. These images contain a vast amount of information, which is often hidden behind various sources of noise, convoluted with other information and stochastic in nature. Accessing the desired biological information therefore requires new tools of computational image analysis and modeling. Here, we review some of the recent advances in computational analysis of images obtained from fluorescence microscopy, focusing on bacterial systems. We emphasize techniques that are readily available to molecular and cell biologists but also point out examples where problem-specific image analyses are necessary. Thus, image analysis is not only a toolkit to be applied to new images but also an integral part of the design and implementation of a microscopy experiment.

PMID:
22415868
PMCID:
PMC4056673
DOI:
10.1002/bies.201100148
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley Icon for PubMed Central
    Loading ...
    Support Center