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Sensors (Basel). 2009;9(5):3549-62. doi: 10.3390/s90503549. Epub 2009 May 13.

Micro-pattern guided adhesion of osteoblasts on diamond surfaces.

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1
Institute of Physics, Academy of Sciences of the Czech Republic, Cukrovarnicka 10, Prague 6, Czech Republic; E-Mail: ukraintsev@fzu.cz ; kromka@fzu.cz.

Abstract

Microscopic chemical patterning of diamond surfaces by hydrogen and oxygen surface atoms is used for self-assembly of human osteoblastic cells into micro-arrays. The cell adhesion and assembly is further controlled by concentration of cells (2,500-10,000 cells/cm(2)) and fetal bovine serum (0-15%). The cells are characterized by fluorescence microscopy of actin fibers and nuclei. The serum protein adsorption is studied by atomic force microscopy (AFM). The cells are arranged selectively on O-terminated patterns into 30-200 μm wide arrays. Higher cell concentrations allow colonization of unfavorable H-terminated regions due to mutual cell communication. There is no cell selectivity without the proteins in the medium. Based on the AFM, the proteins are present on both H- and O-terminated surfaces. Pronounced differences in their thickness, surface roughness, morphology, and phase images indicate different conformation of the proteins and explain the cell selectivity.

KEYWORDS:

atomic force microscopy; biosensors; biotechnology; cell adhesion; diamond; osteoblasts; proteins; tissue engineering

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