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Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):4816-21. doi: 10.1073/pnas.1201448109. Epub 2012 Mar 12.

Tfb6, a previously unidentified subunit of the general transcription factor TFIIH, facilitates dissociation of Ssl2 helicase after transcription initiation.

Author information

1
Department of Structural Biology, Stanford University, Stanford, CA 94305, USA.

Abstract

General transcription factor TFIIH, previously described as a 10-subunit complex, is essential for transcription and DNA repair. An eleventh subunit now identified, termed Tfb6, exhibits 45% sequence similarity to human nuclear mRNA export factor 5. Tfb6 dissociates from TFIIH as a heterodimer with the Ssl2 subunit, a DNA helicase that drives promoter melting for the initiation of transcription. Tfb6 does not, however, dissociate Ssl2 from TFIIH in the context of a fully assembled transcription preinitiation complex. Our findings suggest a dynamic state of Ssl2, allowing its engagement in multiple cellular processes.

PMID:
22411836
PMCID:
PMC3323989
DOI:
10.1073/pnas.1201448109
[Indexed for MEDLINE]
Free PMC Article

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