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Nat Nanotechnol. 2012 Mar 11;7(4):257-63. doi: 10.1038/nnano.2012.24.

Stochastic sensing of proteins with receptor-modified solid-state nanopores.

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1
Walter Schottky Institute & Institute for Advanced Study, Technische Universität München, Am Coulombwall 4, 85748 Garching, Germany.

Abstract

Solid-state nanopores are capable of the label-free analysis of single molecules. It is possible to add biochemical selectivity by anchoring a molecular receptor inside the nanopore, but it is difficult to maintain single-molecule sensitivity in these modified nanopores. Here, we show that metallized silicon nitride nanopores chemically modified with nitrilotriacetic acid receptors can be used for the stochastic sensing of proteins. The reversible binding and unbinding of the proteins to the receptors is observed in real time, and the interaction parameters are statistically analysed from single-molecule binding events. To demonstrate the versatile nature of this approach, we detect His-tagged proteins and discriminate between the subclasses of rodent IgG antibodies.

PMID:
22406921
DOI:
10.1038/nnano.2012.24
[Indexed for MEDLINE]
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