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J Clin Microbiol. 2012 May;50(5):1783-6. doi: 10.1128/JCM.00310-12. Epub 2012 Mar 7.

Real-time PCR assay for identification of Blastomyces dermatitidis in culture and in tissue.

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1
Mycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, USA.

Abstract

The TaqMan real-time PCR assay was developed from the Blastomyces dermatitidis BAD1 gene promoter. The assay identified all haplotypes of B. dermatitidis and five of six positive paraffin-embedded tissues. The assay sensitivity threshold was 1 pg genomic DNA of the mold form and 2 CFU of the yeast form of B. dermatitidis. No cross-reactivity was observed against other fungal DNA. The assay allowed rapid (5-h) identification of B. dermatitidis from culture and from clinical specimens.

PMID:
22403418
PMCID:
PMC3347106
DOI:
10.1128/JCM.00310-12
[Indexed for MEDLINE]
Free PMC Article
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