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Sensors (Basel). 2010;10(5):4739-55. doi: 10.3390/s100504739. Epub 2010 May 10.

Biosensing for the environment and defence: aqueous uranyl detection using bacterial surface layer proteins.

Author information

1
Biosensors and Biocatalysis Group, Institute of Membranes and Systems Biology, University of Leeds, Leeds, LS2 9JT, UK. sms4dc@leeds.ac

Abstract

The fabrication of novel uranyl (UO(2)(2+)) binding protein based sensors is reported. The new biosensor responds to picomolar levels of aqueous uranyl ions within minutes using Lysinibacillus sphaericus JG-A12 S-layer protein tethered to gold electrodes. In comparison to traditional self assembled monolayer based biosensors the porous bioconjugated layer gave greater stability, longer electrode life span and a denser protein layer. Biosensors responded specifically to UO(2)(2+) ions and showed minor interference from Ni(2+), Cs(+), Cd(2+) and Co(2+). Chemical modification of JG-A12 protein phosphate and carboxyl groups prevented UO(2)(2+) binding, showing that both moieties are involved in the recognition to UO(2)(2+).

KEYWORDS:

S-layer; impedance spectroscopy; metal ion; protein biosensor; sequestering; surface layer; uranium; uranyl

PMID:
22399904
PMCID:
PMC3292144
DOI:
10.3390/s100504739
[Indexed for MEDLINE]
Free PMC Article
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