Send to

Choose Destination
Biochem Cell Biol. 2012 Jun;90(3):397-404. doi: 10.1139/o2012-004. Epub 2012 Mar 7.

Protection of ceruloplasmin by lactoferrin against hydroxyl radicals is pH dependent.

Author information

Institute for Experimental Medicine, N-W Branch of the Russian Academy of Medical Sciences, 197376 Saint-Petersburg, Acad. Pavlov street 12, Russia.


Destruction of ceruloplasmin (Cp) in the presence of hydrogen peroxide is accompanied by the release of the protein's copper ions that provoke formation of hydroxyl radicals (OH˙) and, consequently, further degradation of the protein. Under such conditions, degradation of Cp is hampered by a number of substances able to bind copper ions. Lactoferrin (Lf) is the most active protector of Cp, its protective effect depending on the pH of the medium. The best protection of Cp by Lf was detected at pH 7.4. In an acidic buffer (pH 5.5), Lf did not affect the destruction of Cp. The pH-dependent efficiency of copper binding by Lf is in good agreement with its capacity to protect Cp against degradation provoked by hydrogen peroxide. It seems likely that peroxide-dependent degradation of Cp stimulated by its own copper ions is a part of neutrophil-induced antimicrobial reactions and may take place properly at the foci of inflammation. Interaction of Lf with Cp may regulate the generation of OH˙ from hydrogen peroxide in the foci of inflammation and protect the adjacent tissues.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center