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PLoS One. 2012;7(3):e32707. doi: 10.1371/journal.pone.0032707. Epub 2012 Mar 2.

Feeder cells support the culture of induced pluripotent stem cells even after chemical fixation.

Author information

1
Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, Wako-shi, Saitama, Japan.

Abstract

Chemically fixed mouse embryonic fibroblasts (MEFs), instead of live feeder cells, were applied to the maintenance of mouse induced pluripotent stem (miPS) cells. Formaldehyde and glutaraldehyde were used for chemical fixation. The chemically fixed MEF feeders maintained the pluripotency of miPS cells, as well as their undifferentiated state. Furthermore, the chemically fixed MEF feeders were reused several times without affecting their functions. These results indicate that chemical fixation can be applied to modify biological feeders chemically, without losing their original functions. Chemically fixed MEF feeders will be applicable to other stem cell cultures as a reusable extracellular matrix candidate that can be preserved on a long-term basis.

PMID:
22396791
PMCID:
PMC3292564
DOI:
10.1371/journal.pone.0032707
[Indexed for MEDLINE]
Free PMC Article

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