Format

Send to

Choose Destination
PLoS One. 2012;7(3):e32693. doi: 10.1371/journal.pone.0032693. Epub 2012 Mar 1.

Monodansylpentane as a blue-fluorescent lipid-droplet marker for multi-color live-cell imaging.

Author information

1
Institute of Biological Chemistry, Academia Sinica, National Taiwan University, Taipei, Taiwan.

Abstract

Lipid droplets (LDs) are dynamic cellular organelles responsible for the storage of neutral lipids, and are associated with a multitude of metabolic syndromes. Here we report monodansylpentane (MDH) as a high contrast blue-fluorescent marker for LDs. The unique spectral properties make MDH easily combinable with other green and red fluorescent reporters for multicolor fluorescence imaging. MDH staining does not apparently affect LD trafficking, and the dye is extraordinarily photo-stable. Taken together MDH represents a reliable tool to use for the investigation of dynamic LD regulation within living cells using fluorescence microscopy.

PMID:
22396789
PMCID:
PMC3291611
DOI:
10.1371/journal.pone.0032693
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center