Format

Send to

Choose Destination
Angew Chem Int Ed Engl. 2012 Apr 16;51(16):3969-72. doi: 10.1002/anie.201107813. Epub 2012 Mar 6.

Development of an amine dehydrogenase for synthesis of chiral amines.

Author information

1
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Petit Institute of Bioengineering and Bioscience, Atlanta, GA 30332, USA.

Abstract

A leucine dehydrogenase has been successfully altered through several rounds of protein engineering to an enantioselective amine dehydrogenase. Instead of the wild-type α-keto acid, the new amine dehydrogenase now accepts the analogous ketone, methyl isobutyl ketone (MIBK), which corresponds to exchange of the carboxy group by a methyl group to produce chiral (R)-1,3-dimethylbutylamine.

PMID:
22396126
DOI:
10.1002/anie.201107813
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center