Format

Send to

Choose Destination
Chemphyschem. 2012 Mar;13(4):1079-86. doi: 10.1002/cphc.201100814. Epub 2012 Mar 5.

Hidden Markov analysis of trajectories in single-molecule experiments and the effects of missed events.

Author information

1
Physik Department E22, Technische Universität München, James-Franck-Str, 85748 Garching, Germany. johannes.stigler@ph.tum.de

Abstract

The ever more complex fluctuation patterns discovered by single molecule experiments require statistical methods to analyze multi-state hopping traces of long lengths. Hidden Markov modeling is a statistical tool that offers the scalability to analyze even complex data and extract kinetic information. We give an introduction on how to implement hidden Markov modeling for the analysis of single molecule force spectroscopic traces, deal with missed events, and test the method on a calcium binding protein.

PMID:
22392881
DOI:
10.1002/cphc.201100814

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center