Estimation of the subunit stoichiometry of the membrane-associated daptomycin oligomer by FRET

Biochim Biophys Acta. 2012 Jul;1818(7):1642-7. doi: 10.1016/j.bbamem.2012.02.019.

Abstract

Daptomycin is a lipopeptide antibiotic that kills Gram-positive bacteria by depolarizing their cell membranes. This antibacterial action of daptomycin is correlated with the formation of membrane-associated oligomers. We here examine the number of subunits contained in one oligomer using fluorescence resonance energy transfer (FRET). The results suggest that the oligomer contains approximately 6 to 7 subunits, or possibly twice this number if it spans both membrane monolayers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology
  • Cell Membrane / chemistry*
  • Cell Membrane / drug effects
  • Daptomycin / chemistry*
  • Daptomycin / pharmacology
  • Fluorescence Resonance Energy Transfer / methods*
  • Gram-Positive Bacteria / drug effects
  • Kinetics
  • Kynurenine / chemistry
  • Lipopeptides / chemistry
  • Lipopeptides / pharmacology
  • Models, Chemical
  • Models, Molecular
  • Protein Multimerization*
  • Protein Subunits / chemistry
  • Protein Subunits / pharmacology

Substances

  • Anti-Bacterial Agents
  • Lipopeptides
  • Protein Subunits
  • Kynurenine
  • Daptomycin