Abstract
Daptomycin is a lipopeptide antibiotic that kills Gram-positive bacteria by depolarizing their cell membranes. This antibacterial action of daptomycin is correlated with the formation of membrane-associated oligomers. We here examine the number of subunits contained in one oligomer using fluorescence resonance energy transfer (FRET). The results suggest that the oligomer contains approximately 6 to 7 subunits, or possibly twice this number if it spans both membrane monolayers.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / pharmacology
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Cell Membrane / chemistry*
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Cell Membrane / drug effects
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Daptomycin / chemistry*
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Daptomycin / pharmacology
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Fluorescence Resonance Energy Transfer / methods*
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Gram-Positive Bacteria / drug effects
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Kinetics
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Kynurenine / chemistry
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Lipopeptides / chemistry
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Lipopeptides / pharmacology
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Models, Chemical
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Models, Molecular
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Protein Multimerization*
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Protein Subunits / chemistry
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Protein Subunits / pharmacology
Substances
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Anti-Bacterial Agents
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Lipopeptides
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Protein Subunits
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Kynurenine
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Daptomycin