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Anal Biochem. 2012 May 15;424(2):137-9. doi: 10.1016/j.ab.2012.02.032. Epub 2012 Mar 1.

On-the-resin N-terminal modification of long synthetic peptides.

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1
Department of Materials Science and Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA.

Abstract

Here we present a highly efficient protocol for on-the-resin coupling of fluorescent dyes or other functional groups to the N-termini of synthetic peptides prior to cleavage and deprotection. The protocol avoids expensive preactivated dyes and instead employs carboxylated dyes activated by large amounts of coupling reagents. The protocol was used to label peptides with low reactivity such as long hydrophobic peptides and peptides with strong tendencies to form sterically shielding structures or aggregates in solution. In all cases, the yields far exceeded those from commercially available preactivated compounds.

PMID:
22387389
PMCID:
PMC3331795
DOI:
10.1016/j.ab.2012.02.032
[Indexed for MEDLINE]
Free PMC Article
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