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J Appl Microbiol. 2012 May;112(5):900-6. doi: 10.1111/j.1365-2672.2012.05275.x. Epub 2012 Mar 27.

Biodegradation of kraft lignin by a bacterial strain Comamonas sp. B-9 isolated from eroded bamboo slips.

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  • 1School of Metallurgical Science and Engineering, Central South University, Changsha, China.

Abstract

AIMS:

The aim was to obtain evidences for lignin degradation by unicellular bacterium Comamonas sp. B-9.

METHODS AND RESULTS:

Comamonas sp. B-9 was inoculated into kraft lignin-mineral salt medium (KL-MSM) at pH 7·0 and 30°C for 7 days of incubation. The bacterial growth, chemical oxygen demand (COD) reduction, secretion of ligninolytic enzymes and productions of low-molecular-weight compounds revealed that Comamonas sp. B-9 was able to degrade kraft lignin (KL). COD in KL-MSM reduced by 32% after 7 days of incubation. The maximum activities of manganese peroxidase (MnP) of 2903·2 U l(-1) and laccase (Lac) of 1250 U l(-1) were observed at 4th and 6th day, respectively. The low-molecular-weight compounds such as ethanediol, 3, 5-dimethyl-benzaldehyde and phenethyl alcohol were formed in the degradation of KL by Comamonas sp. B-9 based on GC-MS analysis.

CONCLUSIONS:

This study confirmed that Comamonas sp. B-9 could utilize KL as a sole carbon source and degrade KL to low-molecular-weight compounds.

SIGNIFICANCE AND IMPACT OF THE STUDY:

Comamonas sp. B-9 may be useful in the utilization and bioconversion of lignin and lignin-derived aromatic compounds in biotechnological applications. Meanwhile, using Comamonas sp. B-9 in treatment of wastewater in pulp and paper industry is a meaningful work.

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