Format

Send to

Choose Destination
See comment in PubMed Commons below
Magn Reson Med. 2013 Jan;69(1):255-62. doi: 10.1002/mrm.24216. Epub 2012 Feb 28.

Cell motility of neural stem cells is reduced after SPIO-labeling, which is mitigated after exocytosis.

Author information

1
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

Abstract

MRI is used for tracking of superparamagnetic iron oxide (SPIO)-labeled neural stem cells. Studies have shown that long-term MR tracking of rapidly dividing cells underestimates their migration distance. Time-lapse microscopy of random cellular motility and cell division was performed to evaluate the effects of SPIO-labeling on neural stem cell migration. Labeled cells divided symmetrically and exhibited no changes in cell viability, proliferation, or apoptosis. However, SPIO-labeling resulted in decreased motility of neural stem cells as compared with unlabeled controls. When SPIO-labeled neural stem cells and human induced pluripotent stem cells were transplanted into mouse brain, rapid exocytosis of SPIO by live cells was observed as early as 48 h postengraftment, with SPIO-depleted cells showing the farthest migration distance. As label dilution is negligible at this early time point, we conclude that MRI underestimation of cell migration can also occur as a result of reduced cell motility, which appears to be mitigated following SPIO exocytosis.

PMID:
22374813
PMCID:
PMC3366052
DOI:
10.1002/mrm.24216
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley Icon for PubMed Central
    Loading ...
    Support Center