Nanopore detection of single molecule RNAP-DNA transcription complex

Nano Lett. 2012 Mar 14;12(3):1157-64. doi: 10.1021/nl3002827. Epub 2012 Mar 1.

Abstract

In the past decade, a number of single-molecule methods have been developed with the aim of investigating single protein and nucleic acid interactions. For the first time we use solid-state nanopore sensing to detect a single E. coli RNAP-DNA transcription complex and single E. coli RNAP enzyme. On the basis of their specific conductance translocation signature, we can discriminate and identify between those two types of molecular translocations and translocations of bare DNA. This opens up a new perspectives for investigating transcription processes at the single-molecule level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • DNA, Bacterial / chemistry*
  • DNA, Bacterial / ultrastructure*
  • DNA-Directed RNA Polymerases / chemistry*
  • DNA-Directed RNA Polymerases / ultrastructure*
  • Nanostructures / chemistry*
  • Nanostructures / ultrastructure*
  • Porosity*
  • Protein Binding
  • Transcriptional Activation*

Substances

  • DNA, Bacterial
  • DNA-Directed RNA Polymerases