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Antimicrob Agents Chemother. 2012 May;56(5):2767-9. doi: 10.1128/AAC.06122-11. Epub 2012 Feb 27.

Genomewide overexpression screen for fosfomycin resistance in Escherichia coli: MurA confers clinical resistance at low fitness cost.

Author information

1
Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Madrid, Spain.

Abstract

To determine whether the overexpression of chromosomal genes can confer fosfomycin resistance, genomewide screening of a complete set of 5,272 plasmid-expressed open reading frames of Escherichia coli (ASKA collection) was performed. Major results are that (i) no clinical level of resistance is achieved by overexpressing chromosomal genes, except murA; (ii) this level is reached at a low fitness cost; and (iii) this cost is much lower than that imposed by other mutations conferring fosfomycin resistance.

PMID:
22371901
PMCID:
PMC3346583
DOI:
10.1128/AAC.06122-11
[Indexed for MEDLINE]
Free PMC Article

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