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World J Biol Chem. 2012 Feb 26;3(2):34-40. doi: 10.4331/wjbc.v3.i2.34.

Modulation of heat shock protein response in SH-SY5Y by mobile phone microwaves.

Author information

  • 1Emanuele Calabrò, Salvatore Magazù, Department of Physics, University of Messina, 98166 Messina, Italy.

Abstract

AIM:

To investigate putative biological damage caused by GSM mobile phone frequencies by assessing electromagnetic fields during mobile phone working.

METHODS:

Neuron-like cells, obtained by retinoic-acid-induced differentiation of human neuroblastoma SH-SY5Y cells, were exposed for 2 h and 4 h to microwaves at 1800 MHz frequency bands.

RESULTS:

Cell stress response was evaluated by MTT assay as well as changes in the heat shock protein expression (Hsp20, Hsp27 and Hsp70) and caspase-3 activity levels, as biomarkers of apoptotic pathway. Under our experimental conditions, neither cell viability nor Hsp27 expression nor caspase-3 activity was significantly changed. Interestingly, a significant decrease in Hsp20 expression was observed at both times of exposure, whereas Hsp70 levels were significantly increased only after 4 h exposure.

CONCLUSION:

The modulation of the expression of Hsps in neuronal cells can be an early response to radiofrequency microwaves.

KEYWORDS:

Apoptosis; Electromagnetic field; Heat shock proteins; Microwaves; SH-SY5Y differentiated cells

PMID:
22371824
PMCID:
PMC3286792
DOI:
10.4331/wjbc.v3.i2.34
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