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Anal Biochem. 2012 May 15;424(2):124-6. doi: 10.1016/j.ab.2012.02.015. Epub 2012 Feb 25.

Removing endotoxin from plasmid samples by Triton X-114 isothermal extraction.

Author information

1
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, China.

Abstract

Triton X-114 temperature transition extraction has been considered to be a simple and cost-effective strategy to eliminate endotoxin from plasmid preparations. However, a repeated cooling-heating process may promote the degradation of plasmid DNA. Based on the finding that the cloud point of Triton X-114 solution increases substantially in the presence of small amounts of sodium dodecyl sulfate (SDS) and that electrolytes decrease the cloud point of Triton X-114-SDS solution drastically, we designed a Triton X-114 isothermal extraction method for removing endotoxin from plasmid samples and found that it has the same endotoxin removal efficiency when compared with the temperature transition extraction method.

PMID:
22370278
DOI:
10.1016/j.ab.2012.02.015
[Indexed for MEDLINE]

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