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Antioxid Redox Signal. 2012 Aug 1;17(3):505-12. doi: 10.1089/ars.2012.4574. Epub 2012 Apr 10.

Can we see PIP(3) and hydrogen peroxide with a single probe?

Author information

1
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.

Abstract

A genetically encoded sensor for parallel measurements of phosphatidylinositol 3-kinase activity and hydrogen peroxide (H(2)O(2)) levels (termed PIP-SHOW) was developed. Upon elevation of local phosphatidylinositol 3,4,5-trisphosphate (PIP(3)) concentration, the sensor translocates from the cytosol to the plasma membrane, while a ratiometric excitation change rapidly and simultaneously reports changes in the concentration of H(2)O(2). The dynamics of PIP(3) and H(2)O(2) generation were monitored in platelet-derived growth factor-stimulated fibroblasts and in T-lymphocytes after formation of an immunological synapse. We suggest that PIP-SHOW can serve as a prototype for many fluorescent sensors with combined readouts.

PMID:
22369174
DOI:
10.1089/ars.2012.4574
[Indexed for MEDLINE]

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