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Antioxid Redox Signal. 2012 Sep 15;17(6):890-901. doi: 10.1089/ars.2011.4161. Epub 2012 Apr 17.

RNA interference links oxidative stress to the inhibition of heat stress adaptation.

Author information

1
Department of Medical Chemistry, Semmelweis University, Budapest, Hungary.

Abstract

Increased oxidative stress is associated with various diseases and aging, while adaptation to heat stress is an important determinant of survival and contributes to longevity. However, the impact of oxidative stress on heat resistance remains largely unclear.

AIM:

In this study we investigated how oxidative stress impinges on heat stress responses.

RESULTS:

We report that hydrogen-peroxide (H(2)O(2)) pretreatment inhibits both acquired thermotolerance and heat-induced Hsp70 expression in mammalian cells, as well as acquired thermotolerance in the nematode Caenorhabditis elegans, via RNA interference. Moreover, we demonstrate that elimination of RNA interference by silencing key enzymes in microRNA biogenesis, dcr-1 or pash-1, restores the diminished intrinsic thermotolerance of aged and H(2)O(2)-elimination compromised (catalase-2 and peroxiredoxin-2 deficient) worms.

INNOVATION AND CONCLUSION:

These results uncover a novel post-transcriptional element in the regulation of heat stress adaptation under oxidative conditions that may have implications in disease susceptibility and aging.

PMID:
22369044
PMCID:
PMC3392618
DOI:
10.1089/ars.2011.4161
[Indexed for MEDLINE]
Free PMC Article
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