Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2012;864:341-66. doi: 10.1007/978-1-61779-624-1_13.

Extraction of plant secondary metabolites.

Author information

1
Ingredient and Process Research, Kraft Foods, Glenview, IL, USA.

Abstract

This chapter presents an overview of the preparation of extracts from plants using organic solvents, with emphasis on common problems encountered and methods for their reduction or elimination. In addition to generally applicable extraction protocols, methods are suggested for selectively extracting specific classes of plant-derived compounds, and phytochemical procedures are presented for the detection of classes of compounds encountered commonly during extraction, including selected groups of secondary metabolites and interfering compounds. Successful extraction begins with careful selection and preparation of plant samples and thorough review of the appropriate literature for suitable protocols for a particular class of compounds or plant species. During the extraction of plant material, it is important to minimize interference from compounds that may co-extract with the target compounds, and to avoid contamination of the extract, as well as to prevent decomposition of important metabolites or artifact formation as a result of extraction conditions or solvent impurities.

PMID:
22367903
DOI:
10.1007/978-1-61779-624-1_13
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Support Center